![]() ![]() The documentation of plates was performed using TLC visualization device and the images of plates were processed using a digital processor. The proposed method involved the chromatographic separations of xilo-, galacto-, fructo-oligosaccharides mixtures at different molecular weights, tri- and disaccharides (raffinose, sucrose, lactose), and the corresponding monosaccharides (xylose, fructose, galactose) on HPTLC plates, using different eluent mixtures and elution conditions. HPTLC is a widely used, fast and accurate method of separating complex mixtures. ![]() For this reason, the objective of this study is to develop and validate a simple and relatively economical analytical method for detecting complex sugar mixtures in fermentation broth based on High-Performance Thin-Layer Chromatography (HPTLC). A method for detection and quantification of sugar levels are very advantageous because they can be considered as key indicators in determining the yields and the productivity of the process. Visit for more related articles at Journal of Analytical & Bioanalytical TechniquesĬarbohydrates are the principal sources of nutrient and energy in large-scale submerged fermentation processes. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received date: JAccepted date: JPublished date: June 23, 2015Ĭitation: Bernardi T, Pedrini P, Marchetti MG, Tamburini E (2015) Separation and Quantitative Determination of Carbohydrates in Microbial Submerged Cultures Using Different Planar Chromatography Techniques (HPTLC, AMD, OPLC). Tatiana Bernardi 1, Paola Pedrini 2, Maria Gabriella Marchetti 2 and Elena Tamburini 2 *ġ Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, ItalyĢ Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy *Corresponding Author: Elena Tamburiniĭepartment of Life Sciences and BBiotechnology Submerged Cultures Using Different Planar Chromatography Techniques Separation and Quantitative Determination of Carbohydrates in Microbial ![]()
0 Comments
Leave a Reply. |